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CAPILLARYS 2 and MINICAP Immunotyping (IT) Assay
An automated alternative to immunofixation testing

Designed for the detection and characterization of monoclonal proteins in serum utilizing Sebia’s capillary electrophoresis (CE) instruments – CAPILLARYS 2 and MINICAP. Immunotyping is a completely automated assay from bar-coded primary sample tube to the final result report, offering true positive identification. Sample dilutions, standard or nonstandard (hypogamma or hypergamma), are automatically performed by the instrument.

Similar to agarose gel electrophoresis, antisera is utilized; however, no manual antisera pipetting is required. With CAPILLARYS 2, a sealed manufactured antisera cartridge is utilized. With MINICAP, bar-coded antisera vials are placed directly on the carousel. Each serum sample is mixed with individual antisera that are specific against gamma (IgG), alpha (IgA) and mu (IgM) heavy chains, and kappa and lambda (free and bound) light chains.

View an introduction to Immunotyping on CAPILLARYS 2

Immunotyping Methodology
A small amount of sample is mixed with a specific diluent and is then mixed with individual specific antisera. A soluble immune complex is formed rapidly between the Ig and the corresponding antibody; no incubation, sedimentation or centrifugation is required. The large antibody complex exhibits an altered or slowed anodic migration, moving outside of the determined detection window. The untreated reference curve is compared to all of the immunological curves in order to identify and type the monoclonal component present in the sample.


P/N Description
2100 CAPILLARYS Immunotyping
2003 CAPILLARYS Protein 6
2300 MINICAP Immunotyping
2223 MINICAP Protein 6