Perfect for the research laboratory, Apo A1B is designed to provide the simultaneous quantification of apolipoproteins A and B1in serum. Apo A1 is one of the major components of HDL which plays a major role in the transport of cholesterol to the liver for metabolism. Apo B, on the other hand, is a major component of LDL and is the primary lipoprotein group present in atherosclerotic lesions. It follows that the proportional association of these two lipoproteins is of importance in determining an individual's atherosclerotic risk assessment and monitoring.

In this electroimmunodiffusion technique, two monospecific antibodies to Apo A1 and Apo B are incorporated into an agarose gel. Four standards and patient samples are diluted with lipid stain for visualization and then electrophoresed. Based on the principles of Laurel rocket electrophoresis, the result is two superimposed rockets for each sample. The shorter rocket corresponds to the Apo B and the higher rocket corresponds to the Apo A1. Standards for each are plotted and the lipid concentrations calculated.

Sebia's Apo A1B assay is the kit many research scientists choose for its ease of use, clarity of results, fast processing, and excellent correlation to gravimetric methods.