Sebia's ISO-PAL assay is designed to separate the isoenzymes of alkaline phosphatase found in serum. Alkaline Phosphatase is an enzyme normally present in high concentrations in growing bone and in bile, but it is present in many human tissues including liver, bone, kidney, intestine and placenta. Its elevation is seen in a variety of hepatic and non-hepatic conditions as well as in normal adolescent growth. Essential for the deposition of minerals in bones and teeth, it is also present in the blood and is an important clinical diagnostic indicator for several illnesses, including chronic myelogenous leukemia.

The individual AP isoenzymes can be generally separated by electrophoresis; however, the electrophoretic mobilities of the liver and bone isoenzymes are quite similar. In other assays, samples must be pretreated or heat inactivated for good separation. However, with Sebia's ISO-PAL assay there is no sample pretreatment required. With samples run in pairs, lectin reagent is used on one track to separate the liver and bone isoenzymes. Sample migrations are compared of the two side-by-side tracks allowing excellent quantitation of these isoenzymes in just one quick assay.